Germinating Lisianthus (Eustoma), also known as Prairie Gentian, requires specific conditions due to its slow and delicate germination process. Here’s a step-by-step guide to help you successfully germinate Lisianthus seeds:

1. Seed Selection and Preparation

Quality Seeds: Purchase fresh, high-quality seeds from a reputable supplier. Lisianthus seeds are very small, so handle them with care.
Pelleted Seeds: If possible, use pelleted seeds, which are easier to handle and plant.
2. Sowing the Seeds

Timing: Start seeds indoors 10-12 weeks before the last expected frost date. Lisianthus has a long growing season and requires early starting.
Seed Trays: Use seed trays or small pots filled with a sterile, well-draining seed starting mix. Moisten the mix before sowing.
Sowing: Scatter the seeds thinly on the surface of the soil. Do not cover the seeds with soil, as they need light to germinate.
Mist: Gently mist the seeds with water to settle them into the soil.
3. Germination Conditions

Light: Provide bright, indirect light. Using a grow light is ideal if natural light is insufficient.
Temperature: Maintain a consistent temperature between 70-75°F (21-24°C). A heat mat can help maintain the right temperature.
Humidity: Cover the seed tray with a clear plastic cover or plastic wrap to create a humid environment. Ensure good ventilation to prevent mold growth.
4. Germination and Early Growth

Patience: Germination can take 10-20 days. Be patient and keep the conditions consistent.
Watering: Keep the soil consistently moist but not waterlogged. Water from the bottom if possible to avoid disturbing the seeds.
Thinning: Once seedlings have 2-3 true leaves, thin them to avoid overcrowding.
5. Transplanting

First Transplant: When seedlings have several true leaves and are large enough to handle, transplant them into individual pots. Use a light, well-draining potting mix.
Hardening Off: Gradually acclimate the seedlings to outdoor conditions over a week or two before transplanting them into the garden.
Final Transplant: Transplant the seedlings into the garden after all danger of frost has passed. Space them about 6-8 inches apart.
6. Care and Maintenance

Sunlight: Plant in a location that receives full sun to partial shade.
Soil: Ensure the soil is well-draining and fertile. Amend with compost if needed.
Watering: Keep the soil evenly moist but not waterlogged. Mulch can help retain moisture and suppress weeds.
Feeding: Fertilize every 2-4 weeks with a balanced, water-soluble fertilizer to encourage healthy growth and blooming.